Chemistry at EMBL
Köhn Group
Phosphatase Investigation with Chemical Biology Tools
The Köhn Group performs organic synthesis in the fields of (phospho)peptide- and inositol- solid and solution phase chemistry. We also have expertise in peptide and small molecule microarray preparation. The synthesized peptides, inositides and their analogs are applied as substrates and inhibitors for phosphatases using different screening methods. In addition, we perform expressed protein ligation to modify phosphatases in order to study their regulatory mechanisms.
Selected publications
The Metastasis-Promoting Phosphatase PRL-3 Shows Activity toward Phosphoinositides. McParland V, Varsano G, Li X, Thornton J, Baby J, Aravind A, Meyer C, Pavic K, Rios P, Köhn M. Biochemistry. 2011 Sep 6;50(35):7579-7590.
Structure-activity analysis of semisynthetic nucleosomes: mechanistic insights into the stimulation of Dot1L by ubiquitylated histone H2B. McGinty, R.K., Köhn, M., Chatterjee, C., Chiang, K.P., Pratt, M.R. & Muir, T.W. ACS Chem Biol. 2009;4(11):958-968.
A microarray strategy for mapping the substrate specificity of protein tyrosine phosphatase. Köhn, M., Gutierrez-Rodriguez, M., Jonkheijm, P., Wetzel, S., Wacker, R., Schroeder, H., Prinz, H., Niemeyer, C.M., Breinbauer, R., Szedlacsek, S.E. & Waldmann, H. Angew Chem Int Ed Engl. 2007;46(40):7700-7703.
Immobilization strategies for small molecule, peptide and protein microarrays. Köhn, M. J Pept Sci. 2009 Mar 23;15(6):393-397.
Teaching:
Current lectures:
"Chemische Biologie 1", Universität Karlsruhe
Previous lectures:
"Chemische Biologie 1 und 2", Universität Karlsruhe
Chemical biology at EMBL
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