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Staff at EMBL

Sara Buonomo

Staff Scientist
Avner Group

EMBL Monterotondo, Adriano Buzzati-Traverso Campus, Via Ramarini 32, 00016 Monterotondo, Italy

E-mail: send a mail

DNA replication and genome stability
The creation of two identical copies of the genome is the defining event of life, essential for cell division. This process takes place during Synthesis-phase of the cell cycle. DNA replication is a very stressful event, as mistakes can lead to mutations and any obstacle on the way of the replication machinery could cause irreparable damage. Problems during DNA replication are the most frequent initiating events in cancer development. To control the fidelity of this process cells have evolved checkpoint mechanisms. Moreover, DNA replication itself is highly organized, both spatially and temporally inside the nucleus. While the function of numerous proteins has been elucidated in the monitoring of replication and in the signaling of arising problems, very little is known about the factors involved in the spatio-temporal organization of the DNA replication process.


Publications

Structural and Biophysical Characterization of Murine Rif1 C Terminus Reveals High Specificity for DNA Cruciform Structures. Sukackaite, R., Jensen, M.R., Mas, P.J., Blackledge, M., Buonomo, S.B. & Hart, D.J. J Biol Chem. 2014 Mar 14. Europe PMC

53BP1 regulates DSB repair using Rif1 to control 5' end resection. Zimmermann, M., Lottersberger, F., Buonomo, S.B., Sfeir, A. & de Lange, T. Science. 2013 Feb 8;339(6120):700-4. doi: 10.1126/science.1231573. Epub 2013 Jan10. Europe PMC

Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells. Cornacchia, D., Dileep, V., Quivy, J.P., Foti, R., Tili, F., Santarella-Mellwig, R., Antony, C., Almouzni, G., Gilbert, D.M. & Buonomo, S.B. EMBO J. 2012 Jul 31;31(18):3678-90. doi: 10.1038/emboj.2012.214. Epub 2012 Jul31. Europe PMC

Heterochromatin DNA replication and Rif1. Buonomo, S.B. Exp Cell Res. 2010 Jul 15;316(12):1907-13. Epub 2010 Mar 25. Europe PMC

Mammalian Rif1 contributes to replication stress survival and homology-directed repair. Buonomo, S.B., Wu, Y., Ferguson, D. & de Lange, T. J Cell Biol. 2009 Nov 2;187(3):385-98. doi: 10.1083/jcb.200902039. Europe PMC

Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Silverman, J., Takai, H., Buonomo, S.B., Eisenhaber, F. & de Lange, T. Genes Dev. 2004 Sep 1;18(17):2108-19. Europe PMC

Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19. Buonomo, S.B., Rabitsch, K.P., Fuchs, J., Gruber, S., Sullivan, M., Uhlmann, F., Petronczki, M., Toth, A. & Nasmyth, K. Dev Cell. 2003 May;4(5):727-39. Europe PMC

Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin. Buonomo, S.B., Clyne, R.K., Fuchs, J., Loidl, J., Uhlmann, F. & Nasmyth, K. Cell. 2000 Oct 27;103(3):387-98. Europe PMC

A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Klein, F., Mahr, P., Galova, M., Buonomo, S.B., Michaelis, C., Nairz, K. & Nasmyth, K. Cell. 1999 Jul 9;98(1):91-103. Europe PMC