Gene Expression ServiceLinks
General information on Recombineering
Selected BAC sources
- Childrens Hospital Oakland Research Institute / BACPAC (RP23 and RP24 BAC libraries)
- Empire Genomics (RP11, RP22 and RP23 BAC libraries)
- ImaGenes (RP23 BAC library)
- Invitrogen (RP22 and RP23 BAC libraries)
- MSMG BACs are available from Dr. Abe/RIKEN
Selected literature on Red/ET recombination and DNA manipulation
Original Articles
An improved recombineering approach by adding RecA to lambda Red recombination.
Wang J, Sarov M, Rientjes J, Fu J, Hollak H, Kranz H, Xie W, Stewart AF, Zhang Y.
Mol Biotechnol. 2006 Jan;32(1):43-53.
A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.
Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF.
Nat Methods. 2006 Oct;3(10):839-44.
BAC engineering for the generation of ES cell-targeting constructs and mouse transgenes.
Testa G, Vintersten K, Zhang Y, Benes V, Muyrers JP, Stewart AF.
Methods Mol Biol. 2004;256:123-39.
A highly efficient recombineering-based method for generating conditional knockout mutations.
Liu P, Jenkins NA, Copeland NG.
Genome Res. 2003 Mar;13(3):476-84.
Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles.
Testa G, Zhang Y, Vintersten K, Benes V, Pijnappel WW, Chambers I, Smith AJ, Smith AG, Stewart AF.
Nat Biotechnol. 2003 Apr;21(4):443-7.
Point mutation of bacterial artificial chromosomes by ET recombination.
Muyrers JP, Zhang Y, Benes V, Testa G, Ansorge W, Stewart AF.
EMBO Rep. 2000 Sep;1(3):239-43.
DNA cloning by homologous recombination in Escherichia coli.
Zhang Y, Muyrers JP, Testa G, Stewart AF.
Nat Biotechnol. 2000 Dec;18(12):1314-7.
A new logic for DNA engineering using recombination in Escherichia coli.
Zhang Y, Buchholz F, Muyrers JP, Stewart AF.
Nat Genet. 1998 Oct;20(2):123-8.
Review Articles
Current issues in mouse genome engineering.
Glaser S, Anastassiadis K, Stewart AF.
Nat Genet. 2005 Nov;37(11):1187-93.
Recombineering: a powerful new tool for mouse functional genomics.
Copeland NG, Jenkins NA, Court DL.
Nat Rev Genet. 2001 Oct;2(10):769-79.
Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA.
Muyrers JP, Zhang Y, Stewart AF.
Trends Biochem Sci. 2001 May;26(5):325-31.
ET-cloning: think recombination first.
Muyrers JP, Zhang Y, Stewart AF.
Genet Eng (N Y). 2000;22:77-98.
Technical Reports and Protocols
Recombineering: genetic engineering in bacteria using homologous recombination.
Thomason L, Court DL, Bubunenko M, Costantino N, Wilson H, Datta S, Oppenheim A.
Curr Protoc Mol Biol. 2007 April; Chapter 1: Unit 1.16.
BAC engineering for the generation of ES cell-targeting constructs and mouse transgenes.
Testa G, Vintersten K, Zhang Y, Benes V, Muyrers JP, Stewart AF.
Methods Mol Biol. 2004;256:123-39.
ET recombination: DNA engineering using homologous recombination in E. coli.
Muyrers JP, Zhang Y, Benes V, Testa G, Rientjes JM, Stewart AF.
Methods Mol Biol. 2004;256:107-21.