The Genome Engineering Service at EMBL Monterotondo provides DNA targeting constructs primarily for the generation of genetically modified mice.

We assemble large-sized DNA constructs not using conventional cloning but rather alter DNA molecules by homologous recombination based on the lambda Red/ET recombination system, a DNA engineering technique that was termed “Recombineering”. Hereby, recombination occurs through user-defined homology regions so that any position of a target DNA molecule can be altered without having to rely on restriction endonucleases and even without leaving unwanted sequences.

Main activities of the Genome Engineering Service comprise the design and generation of targeting constructs for general and conditional knockout or knock-in strategies, the construction of custom-defined transgenic cassettes, and the engineering of large BAC transgenic constructs. We provide experience in the conceptual design of the targeting construct and subsequently perform the actual engineering. As a source of genomic DNA we use human and mouse BAC clones, and besides our collection of pre-assembled cassettes containing selectable markers we offer the service to also construct custom-made insertion cassettes.