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Transgenic FacilityServices

At the Transgenic Mouse Facility of EMBL-Monterotondo the following services are available. For additional information please select.

Production of Transgenic Mice

Microinjection of foreign DNA or lentivirral vectors into fertilized oocytes of inbred or hybrid mouse strains.

  • Pronuclear injection of DNA
  • ICSI-Mediated Gene Transfer

Production of Chimeric Mice

  • Injection of targeted ES cells into host 8 cell stage embryos
  • Injection of targeted ES cells into host blastocyst stage embryos

Line Cryopreservation and Revival

Cryopreservation is one of the most reliable techniques to maintain genetically modified mouse strains.

  • Cryopreservation of embryos Cryopreservation of Sperm