Seminar Colour Guide:              
External Faculty Speaker
Thursday, 28 September 2017, 11:00Add to calendarTo be announcedMiho Nakajima, The Neuroscience Institute Depts. of Psychiatry, Neuroscience and Physiology NYU, Langone Medical Center, USAHost: Hiroki AsariCNR Seminar Room, EMBL Rome
Science and Society
Friday, 29 September 2017, 11:00Add to calendarWorking Together but how? Scientific collaboration in a globalised worldHelga Nowotny, ETH Zurich, SwitzerlandHost: Halldór StefánssonCNR Seminar Room, EMBL Rome
Abstract: Collaboration is in. So are networks and sharing, open science and open access. Global student mobility is at unprecedented levels and so is globally distributed scientific work. Humanity faces enormous challenges that can only be tackled by large-scale, cross-national efforts of collaboration and coordination. Meanwhile, the unrelenting generation of ever more data, waiting to be curated and processed, made interoperable and translated into tangible benefits, continues to transform the organisation of scientific practice and how to manage scientific institutions.

The younger generation of researchers finds itself caught in the ensuing turbulence. The geographical-spatial extension of research is accompanied by a tighter temporal grip exerted locally. Standardisation of career paths favours mainstream thinking while failure, creativity and diversity are celebrated as desirable ideals at the same time. Expectations of being adept at multi-tasking abound while the focus on the tasks ahead becomes more narrow. How to survive such contradictions? How to embrace the uncertainty that comes with it?

I will attempt to discuss how greater reflexive awareness of the enablement and constraints that come with the current transformation can help to better understand the different imaginaries of what working together might mean and entail.
EMBL Distinguished Visitor Lecture
Friday, 6 October 2017, 10:00Add to calendarSwitching genes on and off during erythropoiesisDouglas Higgs, MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford, United KingdomHost: Philip Avner / Christophe LancrinCNR Seminar Room, EMBL Rome
External Faculty Speaker
Friday, 13 October 2017, 11:00Add to calendarTo be announcedAlla Karpova, HHMI Janelia Farm Research Campus, Virginia, USA, USAHost: Cornelius GrossCNR Seminar Room, EMBL Rome
EMBL - Sapienza Lecture
Friday, 3 November 2017, 11:00Add to calendarEpigenetic variation and non-genetic inheritanceAnne Ferguson-Smith, Department of Genetics, University of Cambridge, Cambridge , UK, United KingdomHost: Cornelius Gross / Irene BozzoniSapienza Università di Roma - Aula Odeion - Museo dell'Arte Classica - P.le Aldo Moro, 5 - Roma
External Faculty Speaker
Tuesday, 7 November 2017, 14:00Add to calendarTo be announcedJoost Gribnau, Erasmus MC - dept of Developmental Biology,, Rotterdam, NetherlandsHost: Philip AvnerCNR Seminar Room, EMBL Rome
External Faculty Speaker
Friday, 10 November 2017, 11:00Add to calendarRegulation and Heterogeneity of Dormant Hematopoietic Stem CellsNina Cabezas-Wallscheid, Max-Planck-Institute of Immunobiology and Epigenetics, Freiburg, Germany, GermanyHost: Christophe Lancrin CNR Seminar Room, EMBL Rome
Abstract: Hematopoietic stem cells (HSCs) harbor the capacity to generate a series of multipotent progenitors (MPPs) that differentiate into lineage-committed progenitors and subsequently mature cells. To explore essential HSC features, we recently integrated quantitative proteome, transcriptome, and methylome analyses of five FACS-sorted HSCs and MPP populations (MPP1-4) and combined these OMICs analyses to their functional potential (Cabezas-Wallscheid et al., Cell Stem Cell 2014; Klimmeck et al., Stem Cell Reports 2014; Lipka et al., Cell Cycle 2014). From the characterization of more than 6,000 proteins, 27,000 transcripts, and 15,000 differentially methylated regions (DMRs), we identified coordinated changes associated with early differentiation steps. DMRs show continuous gain or loss of methylation during differentiation, and the overall change in DNA methylation correlates inversely with gene expression at key loci. Our data reveal the differential expression landscape of 493 TFs and 682 lncRNAs and highlight specific expression clusters including Wnt and Lin28-Hmga signaling, the imprinted-gene-network, Hox genes, retinoic acid metabolism. We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells. To address differentiation potential of MPP2-4 we linked our OMICs data with functional reconstitution experiments.
Recently, we have expanded this analysis to dormant HSCs (dHSCs) identified by label-retaining assays (Wilson et al., Cell 2008). Rare dHSCs reside at the top of the blood hierarchy harboring the highest long-term reconstitution capacity. However, till the date the molecular identity of dHSCs, as well as the mechanism regulating maintenance and the transition out of dormancy remain unknown. We now show by single-cell RNA-seq analysis that the transition from dormancy towards cell cycle entry is achieved by a continuous and coordinated up-regulation of all major biosynthetic processes rather than a switch on/off mechanism (Cabezas-Wallscheid et al. Cell 2007). We employed a novel transgenic reporter mouse model that reversibly marks dHSCs avoiding label retention assays. Finally, we show that dietary vitamin A/ retinoic acid signaling is a key pathway for in vivo retaining HSC dormancy.

EMBL Distinguished Visitor Lecture
Friday, 17 November 2017, 10:00Add to calendarEpigenetic mechanisms in early mammalian developmentMaria Elena Torres-Padilla, Institute of Epigenetics and Stem Cells (IES) , Helmholtz Zentrum München, Germany, GermanyHost: Philip Avner CNR Seminar Room, EMBL Rome
Abstract:
External Faculty Speaker
Monday, 11 December 2017, 11:00Add to calendarTo be announcedDéborah Bourc'his , Institut Curie, Unité de Génétique et Biologie du Développement, Paris, FranceHost: Jamie HackettCNR Seminar Room, EMBL Rome
Abstract:
EMBL - Sapienza Lecture
Friday, 12 January 2018, 11:00Add to calendarTransgenerational epigenetic inheritance: Evidence in mammals and potential mechanisms involving the germlineIsabelle Mansuy, University of Zürich and ETH Zürich, Zurich, SwitzerlandHost: Cornelius Gross / Andrea MeleSapienza Università di Roma - Aula Odeion - Museo dell'Arte Classica - P.le Aldo Moro, 5 - Roma, EMBL Rome
External Faculty Speaker
Friday, 23 February 2018, 11:00Add to calendarZebrafish colour vision: Rainbows in the eyesTom Baden, School of Life Sciences, University of Sussex, Brighton, UK, United KingdomHost: Hiroki AsariCNR Seminar Room, EMBL Rome
Abstract: All sensory systems are specialised to efficiently provide information critical within an animal's sensory-ecological niche. In vertebrate vision, the retina of all visual species follows a common set anatomical and functional motifs, yet each species has tweaked details in this network to tune its vision for its natural visual environment. Accordingly, comparing functional processing strategies employed in different species that live in different visual envioronments provides a window into how neuronal networks evolve to better suit novel computational demands. In reference to our existing functional database on the retina of mice, we now study the retina of zebrafish in the context of their natural habitat to address these questions with a particular emphasis on colour vision.
EMBL Distinguished Visitor Lecture
Friday, 9 March 2018, 10:00Add to calendarTo be announcedFrancisco Bezanilla, Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, USAHost: Paul HeppenstallCNR Seminar Room, EMBL Rome
EMBL Distinguished Visitor Lecture
Friday, 6 April 2018, 10:00Add to calendarEpigenomic Signatures of Neuronal Diversity in the Mammalian BrainJoseph Ecker, Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA, USAHost: Cornelius GrossCNR Seminar Room, EMBL Rome
Abstract: DNA methylation is a chemical modification that occurs predominantly on CG dinucleotides in mammalian genomes. However, recent studies from our laboratory have revealed that non-CG methylation (mCH) is more abundant than CG methylation and non-randomly distributed in the genomes of brain cells. mCH accumulates during the establishment of neural circuits and is associated with Rett syndrome. A comprehensive understanding of how neural circuits spanning the entire brain generate the full repertoire of perception and behaviors requires a list of brain cell types, as well the means to target each cell type in order to interrogate the functional interactions that give rise to the emergent properties of the whole system. Neuronal diversity is essential for mammalian brain function but poses a challenge to molecular profiling. To facilitate cell-type-specific epigenomic studies, we have developed approaches to isolate nuclei from subtypes of neocortical neurons, revealing highly distinctive epigenomic landscapes. Hundreds of thousands of regions differ in chromatin accessibility and DNA methylation signatures characteristic of gene regulatory regions which are predicted to bind distinct cohorts of neuron subtype-specific transcription factors. Surprisingly, neuronal epigenomes reflect both past and present gene expression, with DNA hyper-methylation at developmentally critical genes appearing as a novel epigenomic signature in mature neurons. More recently, we have developed single cell methylome profiling methods that now allow an unbiased census of the diversity of neuronal cell types in the mammalian brain. Taken together, these approaches are beginning to link the functional and transcriptional complexity of neurons to their underlying epigenomic diversity.

EMBL Distinguished Visitor Lecture
Friday, 14 September 2018, 10:00Add to calendarTo be announcedJoanna Wysocka , Institute for Stem Cell Biology & Regenerative Medicine, Stanford, California, USAHost: Jamie HackettCNR Seminar Room, EMBL Rome
EMBL Distinguished Visitor Lecture
Friday, 12 October 2018, 10:00Add to calendarEpigenetic regulation by histone acetylationAsifa Akhtar, Max Planck Institute of Immunobiology, and Epigenetics,Freiburg, Germany , , GermanyHost: Philip AvnerCNR Seminar Room, EMBL Rome
Abstract: Our lab is studying the chromatin and epigenetic mechanisms regulated by histone acetylation using evolutionary conserved complexes associated with MOF, a MYST family of histone acetyl transferase. In files and mammals MOF is associated with the MSL and NSL complexes, which are important regulators of gene expression. In flies the MSL complex is well known for regulation of the X chromosome by the process of dosage compensation, while the NSL complex regulates expression of house keeping genes. In mammals, both complexes appear to be involved in regulating diverse cellular processes. The recent progress of our work will be presented.