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DNA replication and genome stability
The creation of two identical copies of the genome is the defining event of life, essential for cell division. This process takes place during Synthesis-phase of the cell cycle. DNA replication is a very stressful event, as mistakes can lead to mutations and any obstacle on the way of the replication machinery could cause irreparable damage. Problems during DNA replication are the most frequent initiating events in cancer development. To control the fidelity of this process cells have evolved checkpoint mechanisms. Moreover, DNA replication itself is highly organized, both spatially and temporally inside the nucleus. While the function of numerous proteins has been elucidated in the monitoring of replication and in the signaling of arising problems, very little is known about the factors involved in the spatio-temporal organization of the DNA replication process.


Total: 15 publication(s)


Sukackaite R, Cornacchia D, Jensen MR, Mas PJ, Blackledge M, Enervald E, Duan G, Auchynnikava T, Köhn M, Hart DJ, Buonomo SBC. (2017)

Mouse Rif1 is a regulatory subunit of protein phosphatase 1 (PP1).

Sci Rep 7(1):2119. doi: 10.1038/s41598-017-01910-1
Europe PMC | doi


Foti R, Gnan S, Cornacchia D, Dileep V, Bulut-Karslioglu A, Diehl S, Buness A, Klein FA, Huber W, Johnstone E, Loos R, Bertone P, Gilbert DM, Manke T, Jenuwein T, Buonomo SCB. (2016)

Nuclear Architecture Organized by Rif1 Underpins the Replication-Timing Program

MOL CELL 61(2):260-273. doi: 10.1016/j.molcel.2015.12.001
Europe PMC | doi


Sukackaite R, Jensen MR, Mas PJ, Blackledge M, Buonomo SB, Hart DJ. (2014)

Structural and Biophysical Characterization of Murine Rif1 C Terminus Reveals High Specificity for DNA Cruciform Structures

J. Biol. Chem. 289(20):13903-13911. doi: 10.1074/jbc.m114.557843
Europe PMC | doi


Zimmermann M, Lottersberger F, Buonomo SB, Sfeir A, de Lange T. (2013)

53BP1 regulates DSB repair using Rif1 to control 5' end resection

Science 339(6120):700-704. doi: 10.1126/science.1231573
Europe PMC | doi


Cornacchia D, Dileep V, Quivy JP, Foti R, Tili F, Santarella-Mellwig R, Antony C, Almouzni G, Gilbert DM, Buonomo SB. (2012)

Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells

EMBO J. 31(18):3678-3690. doi: 10.1038/emboj.2012.214
Europe PMC | doi


Buonomo SB. (2010)

Heterochromatin DNA replication and Rif1

Exp. Cell Res. 316(12):1907-1913. doi: 10.1016/j.yexcr.2010.03.015
Europe PMC | doi


Buonomo SB, Wu Y, Ferguson D, de Lange T. (2009)

Mammalian Rif1 contributes to replication stress survival and homology-directed repair

J. Cell Biol. 187(3):385-398. doi: 10.1083/jcb.200902039
Europe PMC | doi


Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T. (2004)

Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint

Genes Dev. 18(17):2108-2119. doi: 10.1101/gad.1216004
Europe PMC | doi


Buonomo SB, Rabitsch KP, Fuchs J, Gruber S, Sullivan M, Uhlmann F, Petronczki M, Tóth A, Nasmyth K. (2003)

Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19

Dev. Cell 4(5):727-739. doi: 10.1016/s1534-5807(03)00129-1
Europe PMC | doi


Toth A, Rabitsch KP, Galova M, Schleiffer A, Buonomo SBC, Nasmyth K. (2000)

Functional genomics identifies Monopolin: A kinetochore protein required for segregation of homologs during meiosis I

CELL 103(7):1155-1168. doi: 10.1016/S0092-8674(00)00217-8
Europe PMC | doi

Buonomo SB, Clyne RK, Fuchs J, Loidl J, Uhlmann F, Nasmyth K. (2000)

Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin

Cell 103(3):387-398. doi: 10.1016/s0092-8674(00)00131-8
Europe PMC | doi


Buonomo SBC, Michienzi A, De Angelis FG, Bozzoni I. (1999)

The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

RNA 5(8):993-1002. doi: 10.1017/s1355838299990064
Europe PMC | doi

Klein F, Mahr P, Galova M, Buonomo SB, Michaelis C, Nairz K, Nasmyth K. (1999)

A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis

Cell 98(1):91-103. doi: 10.1016/s0092-8674(00)80609-1
Europe PMC | doi

Gonzalez I, Buonomo SBC, Nasmyth K, von Ahsen U. (1999)

ASH1 mRNA localization in yeast involves multiple secondary structural elements and Ash1 protein translation

CURR BIOL 9(6):337-340. doi: 10.1016/S0960-9822(99)80145-6
Europe PMC | doi


Prislei S, Buonomo SBC, Michienzi A, Bozzoni I. (1997)

Use of adenoviral VAI small RNA as a carrier for cytoplasmic delivery of ribozymes

RNA 3(6):677-687.
Europe PMC