The Gross Group is pleased to provide the scientific community with the following recombinant adeno-associated viral (AAV) vectors for neuroscience applications.

These vectors are produced to contain the capsid proteins of both AAV1 and AAV2 for efficient heparin-based purification and broad tropism. For additional information regarding the production and purification procedure, refer to:

Hauck B, Chen L, Xiao W. Generation and characterization of chimeric recombinant AAV vectors. Mol Ther. 2003 Mar;7(3) 419-425. doi:10.1016/s1525-0016(03)00012-1. PMID: 12668138; PMCID: PMC2636682.

McClure C, Cole KL, Wulff P, Klugmann M, Murray AJ. Production and titering of recombinant adeno-associated viral vectors. J Vis Exp. 2011 Nov e3348. doi:10.3791/3348. PMID: 22143312; PMCID: PMC3308604.

Both DNA constructs and AAVs are available upon request. Please address requests for materials and information to Cornelius Gross (gross@embl.it) and Angelo Raggioli (raggioli@embl.it).

Available AAVs

Cytoplasmic and membrane labeling AAVs

ESyn::iRFP670
rSyn::iRFP670
rSyn::iRFP670-farnesyl
hSyn::tdTomato
hSyn::tdTomato-farnesyl

Pre-synaptic labeling AAVs

EF1a::Synaptophysin-GFP
EF1a::Synaptophysin-iRFP

Activity modulating AAVs

CMV::FLEX-hM4D-invVenus
hSyn::Venus-P2A-hM4D

SNAP/Halo expressing AAVs

hSyn::SNAP
hSyn::GPI-SNAP
hSyn::GPI-Halo

Recombinase expressing AAVs

hSyn::Venus-2A-CreERT2

CRISPR AAVs

hSyn::tdTomato; hU6::GFP-targeting sgRNA
hSyn::tdTomato; hU6::empty