Location: Room 42

Description

Automated inverted microscope for biomedical research, ideal for fluorescence

Specifications

Microscope

Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage

Objectives

Magnification

NA

Type

Immersion

Coverslip

WD (mm)

5x

0.15

HCX PL Fluotar

Dry

- / 0.17

 

10x

0.30

HC PL Fluotar

Dry

- / 0.17

11

20x

0.50

HC PL Fluotar

Dry

- / 0.17

1.15

40x

0.75

HCX PL Fluotar

Dry

0.40

0.75

63x

1.4-0.6

HC PL Apo

Oil

0.17

0.60

Fluorescent source

Leica EL6000External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)

Fluorescence filter sets

Name

Excitation filter

Emission filter

Dichroic

A

BP 340 - 380 nm

LP 430 nm

400 nm

CFP

BP 436/20 nm

LP 515 nm

455 nm

L5

BP 480 - 656 nm

LP 590 nm

580 nm

YFP

BP 500/20 nm

BP 535/30  nm

515 nm

N2.1

BP 515 - 560 nm

LP 590 nm

580 nm

Y5

BP 620/60 nm

BP 700/75  nm

Y5

Software

Leica AF6000 64bit Z control and SW Autofocus, multichannel acquisition, stitching

Cameras

  • Leica DFC345 FX C mount 0.55 x HC
  • Leica DFC 450 C mount 0.55 x HC. CCD 2/3" da 2560x1920 px

Power up and down procedure

  • Please contact Alvaro Crevenna to schedule a session
  • Report problems on the Log file

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