leica-LMD7000_s

Location: Room 53

System Specifications

  • Upright fluorescence microscope system combined with a Laser Microdissector
  • Five color excitation filters
  • Suitable for fluorophores from DAPI to Far Red and transmitted image (Brightfield and Phase Contrast).
  • Colored digital camera CD310 (1.3 megapixel)
  • Black and White digital camera CD345
  • Suitable as a laser microdissector for cells and tissue (UV laser).
  • Suitable for imaging fixed specimen (no environmental chamber).

Microscope

Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage

Objectives

Magnification

NA

Type

Immersion

Coverslip

6.3x

0.13

For Microdissection

 

 

10x

0.3

HC PL Fluotar

Dry

- / 0.17

20x

0.4

HCX PL Fluotar

Dry

Correction

40x

0.6

HCX PL Fluotar

Dry

Correction

63x

1.25

HC PL Apo

Oil

0.17

100x

1.32

HC PL Apo

Oil

0.17

Fluorescent source

Leica EL6000External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)

Fluorescence filter sets

Name

Excitation filter

Emission filter

Dichroic

A

BP 340 - 380 nm

LP 430 nm

400 nm

CFP

BP 436/20 nm

LP 515 nm

455 nm

L5

BP 480 - 656 nm

LP 590 nm

580 nm

YFP

BP 500/20 nm

BP 535/30  nm

515 nm

N2.1

BP 515 - 560 nm

LP 590 nm

580 nm

N3

BP 546-12

BP 600/40

565

Y5

BP 620/60 nm

BP 700/75  nm

660 nm

LMD

Combined for Microdissection

 

Softwares

Leica AF6000 64bit Z control and SW Autofocus, multichannel acquisition, stitching. Leica Microdissection software

Cameras

  • Leica DFC345 FX C mount 0.55 x HC
  • Leica DFC 450 C mount 0.55 x HC. CCD 2/3" da 2560x1920 pixel

Power up and down procedure

  • Please contact Alvaro Crevenna to schedule a session
  • Report problems on the Log file

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