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Location: Room 53

System specifications

  • Fast live imaging with low phototoxicity
  • Stand: Nikon Tie with Perfect Focus system
  • XY-stage: motorized
  • Z nanoscan motorized (Prior)
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: Volocity 6.3 for image acquisition, measurements and procesing

Detector

  • EMCCD Camera: Hamamatsu C9100-50 frame transfer
  • Effective number of pixels: 1000 x 1000
  • Cell size: 8.0µm x 8.0µm
  • Effective Area: 8.0mm x 8.0mm
  • Max frame rate: 28.1 frames/second
  • Dynamic range: 14 bit
  • Quantum efficiency (peak): > 60%
  • Read out noise at min EM gain: 10 electrons
  • Read out noise at max EM gain: <1 electron

Laserlines

FIXED wavelengths

Laser type and power

Some dyes and use

405nm

solid state laser, 50 mW

DAPI

445nm

solid state laser, 40 mW

CyanFP

488nm

solid state laser, 50 mW

GFP, Alexa488, CY2, OregonGreen

514nm

solid state laser, 25 mW

YellowFP

561nm

solid state laser, 50 mW

RedFPs, Alexa568

640nm

solid state laser, 40 mW

DRAQ5, CY5, Alexa648

Dichroics

  • 405/488/561/640
  • 405/440/514/640
  • 405/440/514/561

Band pass Filters (Emitters)

Fluorochromes

Bandpass

Emission

Em wheel N.

DAPI / RFP

Dual band

445 /60 - 615 / 70

3

CFP / Far-Red

Dual band

485 /60 - 705 / 90

4

GFP

Single

525 / 55

2

YFP

Single

587 / 125

5

GFP/Far-Red

Dual band

525 / 50 - 640/120

7

Microscope

Nikon CS AFC Bino inverted microscope with motorized XY-stage

Objectives

Magnification

NA

  Type

Immersion

WD (mm)

20x

0.5

Plan Fluor

Dry

2.1

40x

1.3

Plan Fluor

Oil

0.24

40x

1.15

Plan Apo LWD

Water

0.59-0.61

60x

1.4

Plan Fluor

Oil

0.21

100x

1.3

Plan Fluor

Oil

0.20

Fluorescent source

Intensilight external light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)

Fluorescence filter sets (Nikon Microscope Ocular)

Name

Excitation filter

Emission filter

DAPI

BP 340 - 380 nm

BA 435-485 nm

FITC

BP 465 - 495 nm

BA 515-555 nm

G2B

BP 540/25 nm

BA 605/55 nm

Power up and down procedure

  • Please contact Alvaro Crevenna to schedule a session
  • Report problems on the Excel file

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