Location: Room 50

System specifications

  • Dual side illumination
  • Ultrafast light sheet Pivot scan
  • 4 axis sample positioning system
  • Sample Chamber holder and overview camera with LED illumination
  • INDIMO 2971: Preparation for retrofit of optics for cleared samples
  • CO2-Module Lightsheet Z.1 (D) to incubate the Sample Chamber Lightsheet Z.1 with CO2-enriched air. Includes: TempModule CZ-LSFM with accessories TempModule S1 (D)
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: Zen black for image acquisition multiview processing and alignment and deconvolution, Zen blue for measurements and processing
  • “SWAP” 32TB storage and processing workstation with 96GB RAM – ZEN Software incl. Module "Deconvolution"

Detector

  • 2 detection modules: sCMOS PCOedge
  • Effective number of pixels: 1000 x 1000
  • Cell size: 8.0µm x 8.0µm
  • Effective Area: 8.0mm x 8.0mm
  • Max frame rate: 28.1 frames/second
  • Dynamic range: 14 bit
  • Quantum efficiency (peak): > 60%
  • Read out noise at min EM gain: 10 electrons
  • Read out noise at max EM gain: <1 electron

Laserlines

Wavelength (nm)

Laser type and power

Dyes and use

405

solid state laser, 20 mW

DAPI

488

solid state laser, 50 mW

GFP, Alexa488, CY2

561

solid state laser, 20 mW

RedFPs, Alexa568

638

solid state laser, 60 mW

CY5, Alexa647

Band pass Filters

Fluorochromes

Beam Splitter

Filter type

Emission

DAPI / GFP

LP490

Dual

BP 420-70 / BP 505-45

DAPI / RFP

LP510

Dual

BP 420-70 / BP 575-615

GFP / RFP

LP560

Dual

BP 505-45 / BP 575-615

GFP / m-Cherry

LP560

Dual

BP 505-45 / LP 585

GF P/ Far Red

LP560

Dual

BP 505-45 / LP 660

Special optics to generate chromatically corrected light sheets

  • Illumination Optics 5x/0.1 (in combination with 5X detection objectives)
  • Illumination Optics 10x/0.2 (in combination with 20X and 63X detection objectives)

Objectives

Magnification

Type

Immersion

5X

EC Plan-NEOFLUAR 5x/0,16

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20x

W Plan-APO 20x/1,0 UV.Vis

Water

63x

W Plan-APO 63x/1,0 VIS-IR

Water

Power up and down procedure

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